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KMID : 0380020080230010054
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Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 1 p.54 ~ p.58
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The Optimization of Biohydrogen Production Medium by Dark Fermentation with Enterobacter aerogenes
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Choi Young-Jin
Kim Eui-Yong
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Abstract
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Hydrogen is considered as an energy source for the future due to its environmentally friendly use in fuel cells. A promising way is the biological production of hydrogen by fermentation. In this study, the optimization of medium conditions which maximize hydrogen production from Enterobacter aerogenes KCCM 40146 were determined. As a result, the maximum attainable cumulative volume of hydrogen was 431 under the conditions of 0.5M potassium phosphate buffer, pH 6.5 medium containing 30 g/L glucose. The best nitrogen sources were peptone and tryptone for the cell growth as well as hydrogen production. The control of cell growth rate was found to be a important experimental parameter for effective hydrogen production
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KEYWORD
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Biohydrogen production, anaerobe microorganism, dark fermentation, Enterobacter aerogenes KCCM 40146
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